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vivaflow 50r 100 kda mwco tangential flow filtration tff cartridge  (Sartorius AG)


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    Structured Review

    Sartorius AG vivaflow 50r 100 kda mwco tangential flow filtration tff cartridge
    Vivaflow 50r 100 Kda Mwco Tangential Flow Filtration Tff Cartridge, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 96/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kda+tangential+flow+filtration/Vivaflow+50+R+5000+MWCO+Hydrosart/pm41500988-277-8-17
    Average 96 stars, based on 40 article reviews
    vivaflow 50r 100 kda mwco tangential flow filtration tff cartridge - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Formulation:

    Article Title: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models.
    Article Snippet: Pooled fractions were diluted with nuclease-free water to reduce conductivity to <8mS and purified by hydroxyapatite chromatography (Bio-Rad Laboratories, Hercules, CA) to remove unconjugated linkerpayload. .. All FDCs (Table 1) were eluted directly into formulation buffer (100mM disodium phosphate, 100mM sodium chloride, pH 7.2) and concentrated using 10 kDa tangential flow filtration (Vivaflow 200; Sartorius, Göttingen, Germany), followed by 0.22-μm sterile filtration (ThermoFisher Scientific). ..

    Article Title: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models
    Article Snippet: Pooled fractions were diluted with nuclease-free water to reduce conductivity to <8 mS and purified by hydroxyapatite chromatography (Bio-Rad Laboratories, Hercules, CA) to remove unconjugated linker-payload. .. All FDCs (Table ) were eluted directly into formulation buffer (100 mM disodium phosphate, 100 mM sodium chloride, pH 7.2) and concentrated using 10 kDa tangential flow filtration (Vivaflow 200; Sartorius, Göttingen, Germany), followed by 0.22-μm sterile filtration (ThermoFisher Scientific). ..

    Filtration:

    Article Title: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models.
    Article Snippet: Pooled fractions were diluted with nuclease-free water to reduce conductivity to <8mS and purified by hydroxyapatite chromatography (Bio-Rad Laboratories, Hercules, CA) to remove unconjugated linkerpayload. .. All FDCs (Table 1) were eluted directly into formulation buffer (100mM disodium phosphate, 100mM sodium chloride, pH 7.2) and concentrated using 10 kDa tangential flow filtration (Vivaflow 200; Sartorius, Göttingen, Germany), followed by 0.22-μm sterile filtration (ThermoFisher Scientific). ..

    Article Title: Cell-to-cell heterogeneity drives host-virus coexistence in a bloom-forming alga
    Article Snippet: .. After 4 days, the viral lysate was filtered through a 1.2 μm pore size glass microfiber filter (grade GF/C, GE Healthcare Whatman) followed by a 0.45 μm pore size Nalgene Rapid-Flow filter unit (PES, ThermoFisher Scientific) to remove cell debris, before concentrating and washing the virions by 100 kDa tangential flow filtration (Vivaflow 200, Sartorius). .. The concentrated viral lysates were sterile-filtered through a 0.22 μm filter (PVDF, Millex-GV, Millipore) and stored in darkness at 4°C until the infection assay.

    Article Title: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models
    Article Snippet: Pooled fractions were diluted with nuclease-free water to reduce conductivity to <8 mS and purified by hydroxyapatite chromatography (Bio-Rad Laboratories, Hercules, CA) to remove unconjugated linker-payload. .. All FDCs (Table ) were eluted directly into formulation buffer (100 mM disodium phosphate, 100 mM sodium chloride, pH 7.2) and concentrated using 10 kDa tangential flow filtration (Vivaflow 200; Sartorius, Göttingen, Germany), followed by 0.22-μm sterile filtration (ThermoFisher Scientific). ..

    Article Title: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models.
    Article Snippet: .. The ADC1 fractions were pooled and concentrated using 10 kDa tangential flow filtration (Vivaflow 200; Sartorius, Göttingen, Germany), followed by 0.22 μm sterile filtration (ThermoFisher Scientific). .. ADC1 (Table 1) concentration was measured by bicinchoninic acid assay (BCA) using the Rapid Gold BCA kit in accordance with the manufacturer’s recommendations (ThermoFisher Scientific).

    Article Title: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models
    Article Snippet: .. The ADC1 fractions were pooled and concentrated using 10 kDa tangential flow filtration (Vivaflow 200; Sartorius, Göttingen, Germany), followed by 0.22 μm sterile filtration (ThermoFisher Scientific). .. ADC1 (Table ) concentration was measured by bicinchoninic acid assay (BCA) using the Rapid Gold BCA kit in accordance with the manufacturer’s recommendations (ThermoFisher Scientific).

    Sterility:

    Article Title: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models.
    Article Snippet: Pooled fractions were diluted with nuclease-free water to reduce conductivity to <8mS and purified by hydroxyapatite chromatography (Bio-Rad Laboratories, Hercules, CA) to remove unconjugated linkerpayload. .. All FDCs (Table 1) were eluted directly into formulation buffer (100mM disodium phosphate, 100mM sodium chloride, pH 7.2) and concentrated using 10 kDa tangential flow filtration (Vivaflow 200; Sartorius, Göttingen, Germany), followed by 0.22-μm sterile filtration (ThermoFisher Scientific). ..

    Article Title: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models
    Article Snippet: Pooled fractions were diluted with nuclease-free water to reduce conductivity to <8 mS and purified by hydroxyapatite chromatography (Bio-Rad Laboratories, Hercules, CA) to remove unconjugated linker-payload. .. All FDCs (Table ) were eluted directly into formulation buffer (100 mM disodium phosphate, 100 mM sodium chloride, pH 7.2) and concentrated using 10 kDa tangential flow filtration (Vivaflow 200; Sartorius, Göttingen, Germany), followed by 0.22-μm sterile filtration (ThermoFisher Scientific). ..

    Article Title: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models.
    Article Snippet: .. The ADC1 fractions were pooled and concentrated using 10 kDa tangential flow filtration (Vivaflow 200; Sartorius, Göttingen, Germany), followed by 0.22 μm sterile filtration (ThermoFisher Scientific). .. ADC1 (Table 1) concentration was measured by bicinchoninic acid assay (BCA) using the Rapid Gold BCA kit in accordance with the manufacturer’s recommendations (ThermoFisher Scientific).

    Article Title: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models
    Article Snippet: .. The ADC1 fractions were pooled and concentrated using 10 kDa tangential flow filtration (Vivaflow 200; Sartorius, Göttingen, Germany), followed by 0.22 μm sterile filtration (ThermoFisher Scientific). .. ADC1 (Table ) concentration was measured by bicinchoninic acid assay (BCA) using the Rapid Gold BCA kit in accordance with the manufacturer’s recommendations (ThermoFisher Scientific).

    Pore Size:

    Article Title: Cell-to-cell heterogeneity drives host-virus coexistence in a bloom-forming alga
    Article Snippet: .. After 4 days, the viral lysate was filtered through a 1.2 μm pore size glass microfiber filter (grade GF/C, GE Healthcare Whatman) followed by a 0.45 μm pore size Nalgene Rapid-Flow filter unit (PES, ThermoFisher Scientific) to remove cell debris, before concentrating and washing the virions by 100 kDa tangential flow filtration (Vivaflow 200, Sartorius). .. The concentrated viral lysates were sterile-filtered through a 0.22 μm filter (PVDF, Millex-GV, Millipore) and stored in darkness at 4°C until the infection assay.



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    (a) Overview of the experimental workflow, including VLPs production, isolation, delivery, and cargo function assay using reporter cells. (b) Design of candidate sorting domains fused to Cre recombinase via an intein-based self-cleavable linker. Categories include retroviral Gag variants, human Gag homologs (e.g.hArc, hPEG10) and control VSV-G. (c) Schematic of VLPs production by transient transfection and purification via <t>tangential</t> flow <t>filtration</t> (TFF). Intein-mediated autocleavage allows luminal release of Cre cargo. (d) Functional delivery assay using “Traffic Light” (TL) reporter cells containing a LoxP-Red-LoxP-eGFP cassette. Cre delivery induces recombination and eGFP expression, which is detectable by flow cytometry or fluorescence microscopy. (e) Quantification of eGFP⁺ HeLa-TL cells after 48 h incubation with VLPs containing different sorting domains at serial doses (1×10 10 , 10 9 , 10 8 particles/well). All dose–response experiments involving engineered VLPs in cultured cells were carried out in a 96-well plate format, unless indicated otherwise. (f) Functional delivery efficiency (eGFP⁺ %) of selected VLPs (10 8 particles/well) in four TL reporter cell types: HeLa-TL, B16F10-TL, T47D-TL, and MSC-TL. (g) Western blot analysis was performed using lysates from 5×10 5 particle-producing cells and 1×10 10 engineered particles respectively. TSG101, syntenin-1, and β-actin served as particle markers, while calnexin (an endoplasmic reticulum marker) was included to confirm absence of cellular contaminants in particle preparations. Control is the Intein-Cre+VSV-G group. (h) Representative immunofluorescence images of HeLa-TL cells treated with FMLGag-Intein-Cre +VSV-G VLPs or control particles at indicated doses. GFP activation indicates successful delivery. Control is the Cre-loaded particles without VSV-G pseudo tying group. Scale bar, 100 μm. Statistical significance: ****p < 0.0001 .
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    Image Search Results


    (a) Overview of the experimental workflow, including VLPs production, isolation, delivery, and cargo function assay using reporter cells. (b) Design of candidate sorting domains fused to Cre recombinase via an intein-based self-cleavable linker. Categories include retroviral Gag variants, human Gag homologs (e.g.hArc, hPEG10) and control VSV-G. (c) Schematic of VLPs production by transient transfection and purification via tangential flow filtration (TFF). Intein-mediated autocleavage allows luminal release of Cre cargo. (d) Functional delivery assay using “Traffic Light” (TL) reporter cells containing a LoxP-Red-LoxP-eGFP cassette. Cre delivery induces recombination and eGFP expression, which is detectable by flow cytometry or fluorescence microscopy. (e) Quantification of eGFP⁺ HeLa-TL cells after 48 h incubation with VLPs containing different sorting domains at serial doses (1×10 10 , 10 9 , 10 8 particles/well). All dose–response experiments involving engineered VLPs in cultured cells were carried out in a 96-well plate format, unless indicated otherwise. (f) Functional delivery efficiency (eGFP⁺ %) of selected VLPs (10 8 particles/well) in four TL reporter cell types: HeLa-TL, B16F10-TL, T47D-TL, and MSC-TL. (g) Western blot analysis was performed using lysates from 5×10 5 particle-producing cells and 1×10 10 engineered particles respectively. TSG101, syntenin-1, and β-actin served as particle markers, while calnexin (an endoplasmic reticulum marker) was included to confirm absence of cellular contaminants in particle preparations. Control is the Intein-Cre+VSV-G group. (h) Representative immunofluorescence images of HeLa-TL cells treated with FMLGag-Intein-Cre +VSV-G VLPs or control particles at indicated doses. GFP activation indicates successful delivery. Control is the Cre-loaded particles without VSV-G pseudo tying group. Scale bar, 100 μm. Statistical significance: ****p < 0.0001 .

    Journal: bioRxiv

    Article Title: Efficient delivery of gene editors using intein-engineered virus-like particles

    doi: 10.64898/2026.01.25.701600

    Figure Lengend Snippet: (a) Overview of the experimental workflow, including VLPs production, isolation, delivery, and cargo function assay using reporter cells. (b) Design of candidate sorting domains fused to Cre recombinase via an intein-based self-cleavable linker. Categories include retroviral Gag variants, human Gag homologs (e.g.hArc, hPEG10) and control VSV-G. (c) Schematic of VLPs production by transient transfection and purification via tangential flow filtration (TFF). Intein-mediated autocleavage allows luminal release of Cre cargo. (d) Functional delivery assay using “Traffic Light” (TL) reporter cells containing a LoxP-Red-LoxP-eGFP cassette. Cre delivery induces recombination and eGFP expression, which is detectable by flow cytometry or fluorescence microscopy. (e) Quantification of eGFP⁺ HeLa-TL cells after 48 h incubation with VLPs containing different sorting domains at serial doses (1×10 10 , 10 9 , 10 8 particles/well). All dose–response experiments involving engineered VLPs in cultured cells were carried out in a 96-well plate format, unless indicated otherwise. (f) Functional delivery efficiency (eGFP⁺ %) of selected VLPs (10 8 particles/well) in four TL reporter cell types: HeLa-TL, B16F10-TL, T47D-TL, and MSC-TL. (g) Western blot analysis was performed using lysates from 5×10 5 particle-producing cells and 1×10 10 engineered particles respectively. TSG101, syntenin-1, and β-actin served as particle markers, while calnexin (an endoplasmic reticulum marker) was included to confirm absence of cellular contaminants in particle preparations. Control is the Intein-Cre+VSV-G group. (h) Representative immunofluorescence images of HeLa-TL cells treated with FMLGag-Intein-Cre +VSV-G VLPs or control particles at indicated doses. GFP activation indicates successful delivery. Control is the Cre-loaded particles without VSV-G pseudo tying group. Scale bar, 100 μm. Statistical significance: ****p < 0.0001 .

    Article Snippet: The filtered conditioned media were diafiltrated and concentrated to approximately 50 mL by a 300 kDa Tangential flow filtration (TFF, MicroKross, 20 cm 2 , Spectrum laboratories) , .

    Techniques: Isolation, Functional Assay, Retroviral, Control, Transfection, Purification, Filtration, Expressing, Flow Cytometry, Fluorescence, Microscopy, Incubation, Cell Culture, Western Blot, Marker, Immunofluorescence, Activation Assay